View Full Version : pipette
hailey_medschool
02-09-2011, 11:14 AM
do you use automatic pipette or a manual one (with the bulb and all)?
NewfieMike
02-09-2011, 11:29 AM
---> The Lounge
Patho_Medicus
02-09-2011, 11:37 AM
do you use automatic pipette or a manual one (with the bulb and all)?
Wow who uses the bulb pipettes? They're awful! I use automated for macro-scale pipetting I suppose.
Wow who uses the bulb pipettes? They're awful! I use automated for macro-scale pipetting I suppose.
Believe it or not, in gen chem I/II we had to use the bulbs with the long glass tube at UWindsor. It was brutal. Thankfully in 2nd year we graduated to the automatic ones.
proton
02-09-2011, 12:30 PM
My friend worked in a lab in S. America where they were still mouth pipetting :eek:
LostLamb
02-09-2011, 12:46 PM
My friend worked in a lab in S. America where they were still mouth pipetting :eek:
LOL! YIKES!
premed89
02-09-2011, 01:58 PM
I guess it depends on what you're working on, and if you're a grad student or in a student lab... In my chemistry lab now we never use any volume less than 1 mL that needs to be precise.... So we use the manual bulb pipettes which are quite good if you actually know what you're doing...
I mean in biochemistry or biology labs wouldn't you always just use an automatic pipette? Since most volumes are less than 1 mL... I haven't seen any 500 microL buld pipettes :p
Edit: Something like 50 microL would be much better example :P
thatonekid
02-09-2011, 02:13 PM
I'm done with my life of pipettes. But the lab I was in had automatic.
charmer08
02-09-2011, 04:00 PM
I just like to click polls :D
seeking1
02-09-2011, 04:29 PM
The manuel one.
SRS POST: You don't give me the option 'Manual'. :p
Prateek
02-09-2011, 09:12 PM
Pipet Boy = :)
Are we talking about these (http://upload.wikimedia.org/wikipedia/commons/d/d2/Propipette-et-pipette.jpg) and these (http://www.biocomdirect.com/ProductImages/XL3000promo_hr.jpg)? :confused:
charmer08
02-09-2011, 09:38 PM
Are we talking about these (http://upload.wikimedia.org/wikipedia/commons/d/d2/Propipette-et-pipette.jpg) and these (http://www.biocomdirect.com/ProductImages/XL3000promo_hr.jpg)? :confused:
I also like to click on random links :D
dentss
02-09-2011, 09:45 PM
automatic all the way...manual would be so painstaking especially when you have to load up and transfer over 100 samples within a few hrs from one set of pcr tubes to the next!
Corie
02-11-2011, 09:03 PM
Ugh pipettes... I use the manual ones and they drive me crazy. I have to suck up past the line and put my thumb on top and slowly draw down to the line.... Problem is, I always overshoot and can ever get it at the line!
I have heard of those pipettes with up and down valves and was tempted to purchase one even though labs provided pipette bulbs.
Clever
02-11-2011, 09:09 PM
In chemistry labs we use the manual ones; in biology labs we use automatic ones. U of C undergrad is super ghetto.
LostLamb
02-11-2011, 10:06 PM
In chemistry labs we use the manual ones; in biology labs we use automatic ones. U of C undergrad is super ghetto.
Don't worry...all undergrad 'stock labs' use antiquated techniques and equipment...if it isn't 20 years behind the times, then it is too expensive to use in the lab ;)
The Ace of Spades
02-13-2011, 11:27 AM
Pipettes are for chumps! In my research, we measure in beakers, -+5%!
medtech
02-13-2011, 12:40 PM
Pipettes are for chumps! In my research, we measure in beakers, -+5%!
Sign me up!
automatic pipettes are so much easier, I like the repeating kind where you fill the ependorff tube and click for smaller quantities. I can fill a 96 well plate in less than 5 minute :D
TheBoss
02-13-2011, 12:53 PM
Pipettes are for chumps! In my research, we measure in beakers, -+5%!
Umm, wow that really sucks haha. In my undergrad labs we always used manual pipettes, but in actual research they use much better stuff.
The Ace of Spades
02-13-2011, 03:28 PM
Umm, wow that really sucks haha. In my undergrad labs we always used manual pipettes, but in actual research they use much better stuff.
Mind you, my research involved large scale organic synthesis.
emercaughfield
11-16-2012, 03:34 AM
Yes...premed89 is right!!!! It depends on the type of work you are doing. Mostly in schools where we performed experiments just for the purpose of leaning, mouth pipettes are used. But in the major laboratories where main focus is on the precision and accuracy, there bulb pipettes are used to avoid mistakes.
muse87
11-16-2012, 03:52 AM
i used my lab partner, i was more the brains and they were more the hands, lol
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